The Advantages and Characteristics of in plate DLS
- 0.8 – 0.08 µl/well
- Label free
- Standard free
- Non-invasive
- Oxidation protected
- DLS + visuell inspection
- Long term storage
- No cleaning
- Cost efficient
Automated in plate DLS and Imaging
Easy loading is an essential aspect of a smooth workflow. This is facilitated by the use of standard plates. It doesn't matter whether they are loaded manually or robotically. When sealed with paraffin oil, plates can be used very efficiently because they don't follow the loading principle of 96 wells at once, but well by well, even with long pauses.
Sizing and Long term Stability
Monodispersity, absolute size and stability are fundamental indicators of protein integrity.
Imaging and DLS
The combination of imaging and DLS both at the level of designated imaging and DLS systems is unique on the market.
Temperature controlled Plate Hotel
As a hotel system, SpectroQ provides an all-over constant temperature environment for a plate, be it processing, handling, or storage.
Applications (PDF files)
– Automated Imaging
– Automated Repetitive DLS
– Protein Buffer Screening
– Long Term Stability
REFERENCES
Karsten Dierks, Arne Meyer, Howard Einspahr and Christian Betzel
Dynamic Light Scattering in Protein Crystallization Droplets: Adaptations for Analysis and Optimization of Crystallization Processes, Cryst. Growth Des., 2008, 8 (5), pp 1628–1634
Dominik Oberthuer, Emilio Melero-García, Karsten Dierks, Arne Meyer, Christian Betzel, Alfonso Garcia-Caballero and Jose A. Gavira
Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering,
PLoS ONE 7(6): e33545. doi:10.1371/journal.pone.0033545
Arne Meyer, Karsten Dierks, Rana Hussein, Karl Brillet, Hevila Brognaro, and Christian Betzel
Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials,
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 75–81